R&D High Through-Put Enzyme Screening

Accelerate your research by utilizing our ready to use enzyme screening technology

The Substrate Technology

Accelerate Your Research by Utilizing Ready To Use Enzyme Screening Technology

GlycoSpot offers screening technology with two different types of substrates.

Purified Biopolymer Substrates (PBS) are synthesized from pure polysaccharides or proteins.

Complex Biomaterial Substrates (CBS) are substrates synthesized from complex biological material.

Both will work with e.g. purified enzymes, enzyme mixtures, culture broths, plant and animal tissue extracts.

THE SUBSTRATES

PBS

Purified Biopolymer Substrates (PBS)

Synthesized from pure polysaccharides or proteins. Suitable for the detection of polysaccharide degrading enzymes such as glycosyl hydrolases and lyases as well as endo-proteases.

CBS

Complex Biomaterial Substrates (CBS)

Synthesized from complex biological material. Responsive to same types of enzymes as listed above with the specific difference related to the ability of CBS to model complex real-life enzymatic hydrolysis situations.

The Discovery Kit

Discovery
Screening Kit

SUBSTRATES part of GlycoSpot's Discovery Kit

2HE-cellulose

Amylopectin

Amylose

Arabinan

Beta-glucan (barley)

Beta-glucan (yeast)

Curdlan

Dextran

Galactomannan

Pachyman

Pectic galactan

Pullulan

Rhamnogalacturonan

Xylan

Xyloglucan

Chitosan

You Can Bring Down Costs And Accelerate Your Research By Utilizing The High Through-Put Enzyme Screening Technology

Which enzymes do my microorganisms produce? What does my enzyme degrade? What variations do I experience in my consortia of microorganisms? How can I optimize the efficiency of my enzymes?

These are all routine questions in many Research & Development laboratories.

Both in academia and in industry. However, the answer may be less of a routine.

GlycoSpot has developed proprietary technology that can help you screen your enzymes fast and easy in automated setups.

Whether you fancy our existing selection of substrates or prefer custom-made unique substrates, we can be your trusted partner.

What Can It Be Used For

Screening campaigns in microbial consortia
Optimization of bioindustrial processing & products where enzymes are applied
Optimization of enzymatic compositions for e.g. home care, industrial cleaning, textile etc.
Identification & characterization of key enzymatic activities

The Discovery Kit

The Discovery Kit contains 16 different substrates that are perfect for pinpointing the preferred substrate of an enzyme/enzyme mixture whose specificity is unknown. This kit provides an excellent starting point for enzyme discovery and initial evaluation and quanlification.

The Optimization Kit

The Optimization Kit is comprised of your chosen substrate in all 96 multiplate wells and represents a valuable high throughput solution for assessing enzyme concentration/activity/efficiency for enzymes/enzyme mixtures whose specificity is already known.

The Custom-Layout Kit

Design your own enzyme activity screening kit from our long list of PBS and CBS substrates. You can choose your substrates, layout and color in the Custom-Layout Kit. If you like it in a special format, just let us know. The only thing you need to provide are your own enzyme specific solutions.

The Customization Kit

We can make a unique substrate for you, based on material you provide us. Custom-Synthesis Kits allow you to go beyond GlycoSpots standard substrate stock and create your own substrate. We produce unique custom substrates from raw biological material, biomass or purified materials. It allows you to gather specific kinetic information on your enzyme of interest with your substrate.

THE SCREENING KIT

GlycoSpot’s Screening Technology comes in Four Formats: The Discovery Kit, the Optimization Kit, the Custom-Layout Kit and the Customization Kit

THE PLATE READER

GlycoSpot can provide you with a MINI-LAB Tech-Case including a small-sized compact microplate reader called ORION. Portable Plug & Play. Use plate reader at OD 400-1000 nm.

THE SOFTWARE

Use plate reader software to build plate setup and collect data.

Export results (e.g., csv/pdf-format) for data crunching in relevant software using the standard curve of residual activity